Development of a Mimotope Vaccine Targeting the Staphylococcus aureus Quorum Sensing Pathway

Correction: Development of a Mimotope Vaccine Targeting the Staphylococcus aureus Quorum Sensing Pathway

[This corrects the article DOI: 10.1371/journal.pone.0111198.].

Development of a Mimotope Vaccine Targeting the Staphylococcus aureus Quorum Sensing Pathway

A major hurdle in vaccine development is the difficulty in identifying relevant target epitopes and then presenting them to the immune system in a context that mimics their native conformation. We have engineered novel virus-like-particle (VLP) technology that is able to display complex libraries of random peptide sequences on a surface-exposed loop in the coat protein without disruption of pro...

Targeting Staphylococcus aureus Quorum Sensing with Nonpeptidic Small Molecule Inhibitors

A series of 3-oxo-C12-HSL, tetramic acid, and tetronic acid analogues were synthesized to gain insights into the structural requirements for quorum sensing inhibition in Staphylococcus aureus. Compounds active against agr were noncompetitive inhibitors of the autoinducing peptide (AIP) activated AgrC receptor, by altering the activation efficacy of the cognate AIP-1. They appeared to act as neg...

Targeting a Mimotope Vaccine to Activating

Cooperation, quorum sensing, and evolution of virulence in Staphylococcus aureus.

The virulence and fitness in vivo of the major human pathogen Staphylococcus aureus are associated with a cell-to-cell signaling mechanism known as quorum sensing (QS). QS coordinates the production of virulence factors via the production and sensing of autoinducing peptide (AIP) signal molecules by the agr locus. Here we show, in a wax moth larva virulence model, that (i) QS in S. aureus is a ...